Fig. 6.
Effect of iron chase on TfR mRNA stability in primary versus transformed erythroid cells.
Transformed HD3E22 or primary SCF cells were pretreated with 50 M DFO for 24 hours to achieve maximal iron depletion. Subsequently, cells were subjected to an iron chase with 1 mg/mL Tf for the times indicated. (A) After preparation of RNA and Northern blotting, filters were sequentially hybridized with a 32P-labeled probe specific for chicken TfR and for normalization with 18S rRNA. (B) The signals from the autoradiographs were quantitated by PhosphoImage analysis. Also shown are the results from an analogous experiment performed with erythroblasts expressing the v-ErbA oncogene alone.
