Fig. 1.
Down-regulation of Bcl-2, Bcl-xL, and Mcl-1 after treatment by ASOs.
Three HMCLs were studied (L363, U266, and LP-1), and specific ASOs against Bcl-2, Bcl-xL, and Mcl-1 were used. Cells were electroporated without ASO or with either control mismatched or specific ASO. A control consisting of nonelectroporated cells (ie, no treatment) is shown. Cells were recovered at the time corresponding to maximum inhibition of the related protein (18, 40, and 72 hours for Mcl-1, Bcl-xL, and Bcl-2 ASO, respectively). The cells were then lysed, and protein lysates were quantified. Total protein (20 μg) was loaded per lane for immunoblot analysis.
