RT-PCR analyses of KSHV gene expression.

RNA was extracted from LT-BMSCs from 23 patients with MM and 5 healthy donors, and ORF26, 72, and 74 regions of the KSHV genome were amplified. Representative results are shown. Lane 1, the positive control, included RNA from the KSHV-infected BCBL-1 cell line. Lanes 2 to 11 represent RNA from MM patient LT-BMSCs, and lanes 12 to 14 contained RNA from healthy donors.