Relative K5R protein levels in plasma and liver; effect of liver blockage and EDTA injection.

Experiments were performed as described in the legend to Figure 1. Except at 2.4 minutes, the porta hepatis in 2 mice was blocked. A third mouse's liver was not blocked. At 2.5 minutes, EDTA was injected in all 3 mice. Plasma (before EDTA): 10-μL samples were taken from all 3 mice at 2 minutes to determine the plasma concentration of factor IX. Plasma (after EDTA), liver: samples were obtained at 3.5 minutes, 1 minute after EDTA injection.