Fig. 5.
Fig. 5. Hyperproliferative responses of splenic B cells from PECAM-1−/− mice. / T-cell–depleted splenic B cells were purified through Percoll gradient centrifugation and resting B cells recovered from the 75% interface. A total of 105 lymphocytes were cultured in triplicate in microtiter plates in the presence of (A) varying concentrations of LPS, antimouse IgM, and F(ab′)2 fragments of antimouse IgM and (B) varying concentrations of anti-CD40 with or without IL-4 (2 ng/mL) for 72 hours. Proliferation was assessed by the addition of Cell Titre 96 (Promega, Madison, WI) dye reagent for the last 2 hours of culture and absorbance defined at 490 nm.

Hyperproliferative responses of splenic B cells from PECAM-1−/− mice.

T-cell–depleted splenic B cells were purified through Percoll gradient centrifugation and resting B cells recovered from the 75% interface. A total of 105 lymphocytes were cultured in triplicate in microtiter plates in the presence of (A) varying concentrations of LPS, antimouse IgM, and F(ab′)2 fragments of antimouse IgM and (B) varying concentrations of anti-CD40 with or without IL-4 (2 ng/mL) for 72 hours. Proliferation was assessed by the addition of Cell Titre 96 (Promega, Madison, WI) dye reagent for the last 2 hours of culture and absorbance defined at 490 nm.

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