Effect of rhC5a on PAI-1 production in KU-812 cells.
(A) KU-812 cells were incubated for 24 hours in the absence or presence of rhC5a at a concentration of 0.001, 0.01, 0.1, 0.2, and 0.5 μM, respectively. Conditioned media of such treated cells were collected, and total PAI-1 antigen was determined as described in “Materials and methods.” Values are given in ng/106 cells per 24 hours and represent mean values ± SD of 3 independent determinations. Experiments were performed 3 times, and a representative experiment is shown. Total PAI-1 antigen was significantly increased by rhC5a at a concentration greater than 0.1 μM (P < .001). (B) Time-course of the effect of rhC5a on PAI-1 production in KU-812 cells. KU-812 cells were incubated for 4, 8, or 24 hours in the absence (■) or presence of 0.2 μM rhC5a (●). Conditioned media of such treated cells were collected, and total PAI-1 antigen was determined as described in “Materials and methods.” Values are given in ng/106 cells and represent mean values ± SD of 3 independent determinations. Experiments were performed 3 times, and a representative experiment is shown. Total PAI-1 antigen was significantly increased in the presence of rhC5a at all time points tested (P < .001).
