Analysis of the amplification fragments on agarose gels.

(A) Genomic and mRNA structure of the FLT3 gene with position of the primers F11, R12, R5, and R6. (B; top) Ethidium bromide–stained agarose gel with RT-PCR products. (Bottom) cABL control PCR. (C) Classification of FLT3-LM according to intensities of the amplified fragments. Category 1, low intensity; category 2, as intense as wild type; category 3, more intense than wild type; category 4, only mutation; category 5, more than one mutated fragment.