Chemokine receptor expression by B220⁺ DCs.

Semiquantitative RT-PCR analysis of CCR5, CCR6, and CCR7 mRNA expression in control or CpG-treated FACS-sorted thymic B220⁺ DCs. Thymic CD8⁺ DCs, splenic B cells, and anti–CD40-matured epidermal Langerhans (LCs + α-CD40) cells were used as positive controls for the expression of CCR5, CCR6, and CCR7, respectively. RT-PCR was performed on serial dilutions of cDNA from each cell population. β-Actin mRNA levels are shown to control for the relative expression of each cytokine receptor mRNA in the different populations considered.