Modulation of B220⁺ DC function by microbial stimulation.

(A) T-cell stimulation capacity of control or CpG-treated MACS-sorted thymic B220⁺ DCs or CD8⁺ DCs in a 4 day-MLR assay. T-cell proliferation was determined by [³H] thymidine uptake (error bars represent the SD for triplicate cultures). Data are representative of 3 experiments with similar results. (B) IL-12 production by FACS-sorted thymic B220⁺ DCs, thymic CD8⁺ DCs, and peritoneal macrophages (MΦ) after culture in control medium or in the presence of CpG, determined by ELISA (mean ± SD of 3 independent experiments). (C) IL-10 production by FACS-sorted thymic B220⁺ DCs, thymic CD8⁺ DCs, and peritoneal macrophages (MΦ) after culture in control medium or in the presence of CpG, determined by ELISA (mean ± SD of 3 independent experiments).