Fig. 2.
Fig. 2. Phenotypic analysis of B220+ DCs. / (A) Phenotype of thymic B220+ DCs performed on DC-enriched cell fractions. Percentage of cells with fluorescence intensity over the dashed vertical lines, corresponding to the upper limit of control background staining, is indicated. White profiles correspond to the expression of the indicated marker for thymic B220−CD11chi DCs, for which the percentage of cells with fluorescence intensity over the dashed vertical lines is indicated in brackets. Data are representative of 4 experiments with similar results. (B) CD8α expression by B220+CD11cint DCs and B220− CD11chiDCs, in different organs. Percentages of CD8α−, CD8αint, and CD8αhi cells are indicated. Data are representative of 3 experiments with similar results.

Phenotypic analysis of B220+ DCs.

(A) Phenotype of thymic B220+ DCs performed on DC-enriched cell fractions. Percentage of cells with fluorescence intensity over the dashed vertical lines, corresponding to the upper limit of control background staining, is indicated. White profiles correspond to the expression of the indicated marker for thymic B220CD11chi DCs, for which the percentage of cells with fluorescence intensity over the dashed vertical lines is indicated in brackets. Data are representative of 4 experiments with similar results. (B) CD8α expression by B220+CD11cint DCs and B220 CD11chiDCs, in different organs. Percentages of CD8α, CD8αint, and CD8αhi cells are indicated. Data are representative of 3 experiments with similar results.

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