The number of neutrophils in the femur as assessed by FACS analysis is increased after pretreatment with anti–Mac-1 antibodies (5C6).

Mice were pretreated with a single injection of anti–Mac-1 antibodies intraperitoneally (n = 5-18) or saline (n = 5-18) followed by either 5 μg G-CSF per day or saline. The injections were given once daily for 2 days, starting 24 hours after antibody treatment. Twenty-four hours after the last injection, mice were killed and bone marrow was harvested. Results are expressed as mean ± SD. *P < .05 compared with the G-CSF–treated controls.