Fig. 8.
IFN-α killing is dependent on autocrine TNF-α production.
HCs were cultured on polyHEMA in the presence (dotted line) or absence (solid line) of IFN-α (100 U/mL) together with isotype control antibodies (diamonds) or specific anti–TNF-RI- (A; ▪) or anti–TNF-RII- (B; ▪) blocking mAbs. Viability of HCs was determined by FACS analysis of HCs stained with DiOC6 and PI. The same experiment was performed with HCs from another patient, with similar results.
