IFN-α modulates the production of autocrine TNF-α.

Soluble TNF-α was measured by enzyme-linked immunosorbent assay in supernatants of HCs cultured on polyHEMA (A,C) or VN (B,D) in the presence (dotted line) or absence (solid line) of IFN-α (100 U/mL). The results (means ± SEM of triplicate measurements) are from experiments with HCs from 2 different patients.