Fig. 3.
FCS inhibits IFN-α–induced killing of HCs.
Cells were cultured with or without IFN-α (0-100 U/mL as indicated) at a density of 106/mL in 0.5% BSA or 10% FCS on uncoated plastic. Cell lysates were separated by SDS-PAGE and Western blotted for PARP-1. Because HCs adhere to plastic, apoptosis was measured by PARP-1 cleavage because this method, unlike FACS analysis, does not exclude adherent cells from analysis. The results are representative of similar experiments performed with the cells of 4 different HCL patients.
