Fig. 7.
Mapping of tryptic fragments obtained by32P-phosphorylated band 3, untreated or treated with SHP-2.
Isolated erythrocyte membranes were 32P-phosphorylated by Syk and Lyn and incubated in the absence (A) or presence (B) of cytosolic SHP-2 IP, as described. Samples were subjected to SDS-PAGE, and radiolabeled band 3 was excised and digested with trypsin. Resulting 32P-peptides were separated in 2 dimensions on thin-layer cellulose plates and autoradiographed. Arrows indicate sample starting point. Panels represent 3 separate experiments.
