Fig. 1.
TH9402 eliminates proliferating T cells.
(A) CEM T cells were incubated with 10 μM TH 9402 for 40 minutes, washed, and exposed to 5 J/cm2 light after a dye efflux period of 90 minutes. Survival of clonogenic cells for treated (white) and untreated (black) cells was evaluated using a limiting dilution assay. (B) PBMCs exposed to PHA or (C) medium only for 72 hours underwent PDCT. Proliferation to MHC-incompatible cells was evaluated in an MLR at different responder-stimulator (R/S) ratios. Results are expressed as mean ± SEM of experiments performed in triplicate.
