American Society of Hematology

Fig. 6.

$Fig. 6. Western blot analysis of mitochondrial Bax protein in Daudi, Raji, and RL cells after CD40 stimulation. / (A) Daudi cells were cultured in the absence (U) or presence of either 10 μg/mL anti-CD40 antibody (SGN-14 clone, mouse IgG1) (A) or 10 μg/mL srhCD40L (L) for 6 and 48 hours before protein extraction. Protein was extracted by isotonic lysis. Cell lysates using the mitochondrial fractions were analyzed by Western blot for changes in Bax protein. (B) Assessment of Bax protein levels in the mitochondria of Raji cells after anti-CD40 exposure using the SGN-14 antibody and assessed at various time-points. (C) RL cells were cultured in the absence (U) or presence of either 10 μg/mL srhCD40L (L) or 10 μg/mL anti-CD40 SGN-14 (A) for 24 hours. Protein was extracted by isotonic lysis. Cell lysates were analyzed by Western blot for changes in mitochondrial Bax protein. There was an increase in Bax protein after treatment with either ligand or antibody.$

Western blot analysis of mitochondrial Bax protein in Daudi, Raji, and RL cells after CD40 stimulation.

(A) Daudi cells were cultured in the absence (U) or presence of either 10 μg/mL anti-CD40 antibody (SGN-14 clone, mouse IgG1) (A) or 10 μg/mL srhCD40L (L) for 6 and 48 hours before protein extraction. Protein was extracted by isotonic lysis. Cell lysates using the mitochondrial fractions were analyzed by Western blot for changes in Bax protein. (B) Assessment of Bax protein levels in the mitochondria of Raji cells after anti-CD40 exposure using the SGN-14 antibody and assessed at various time-points. (C) RL cells were cultured in the absence (U) or presence of either 10 μg/mL srhCD40L (L) or 10 μg/mL anti-CD40 SGN-14 (A) for 24 hours. Protein was extracted by isotonic lysis. Cell lysates were analyzed by Western blot for changes in mitochondrial Bax protein. There was an increase in Bax protein after treatment with either ligand or antibody.

This Feature Is Available To Subscribers Only