American Society of Hematology

Fig. 5.

$Fig. 5. Western blot analysis of cytosolic Bax protein in RL cells after CD40 stimulation. / RL cells were cultured in the presence or absence of either 10 μg/mL srhCD40L for 48 hours before protein extraction. Protein was extracted by isotonic lysis. Cell lysates containing the cytosolic fraction were analyzed by Western blot using Erk cytosolic protein as a loading control and densitometric analysis for changes in Bax protein relative to the Erk control levels. There was an increase in Bax protein in the cytosolic fractions of RL cells following CD40 stimulation.$

Western blot analysis of cytosolic Bax protein in RL cells after CD40 stimulation.

RL cells were cultured in the presence or absence of either 10 μg/mL srhCD40L for 48 hours before protein extraction. Protein was extracted by isotonic lysis. Cell lysates containing the cytosolic fraction were analyzed by Western blot using Erk cytosolic protein as a loading control and densitometric analysis for changes in Bax protein relative to the Erk control levels. There was an increase in Bax protein in the cytosolic fractions of RL cells following CD40 stimulation.

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