Densitometric analysis of RPA gels.

Quantitative levels of bax, bak, bik, and bclx-S mRNA levels in Daudi cells were established through densitometric analysis of the RPA gel. The gel was exposed to a intensifying screen for 24 hours before analysis on a scanner. Levels of these genes are expressed in ratios to the housekeeping geneGAPDH. There was an increase in all of these gene transcripts compared to the untreated controls. These data are representative of 3 experiments.