Activity of VWF-cleaving protease (ADAMTS13) in 68 patients with thrombocytopenia.

Multimer analysis of VWF substrate digested by diluted (1:20) plasma samples from patients. On each gel (A-H), a calibration curve using dilutions of pooled plasma from healthy donors (1:20 dilution corresponding to 100%) is included. Seventeen patients with severe sepsis (gels A,B), 16 with HIT (gels C,D) and 35 with thrombocytopenia due to various causes (gels E-H) were analyzed. Plasma samples were applied on top of the gel. OMF indicates osteomyelofibrosis; AL, acute leukemia; SAA, severe aplastic anemia; and MISC, miscellaneous.