American Society of Hematology

Fig. 4.

$Fig. 4. Detection of NRAMP1 in human neutrophils granules by Western blotting. / An immunoblot of purified fractions (lane 1, α; lane 2, β1; lane 3, β2; and lane 4, γ; 25 μg of each fraction per lane) was probed with an antibody against NRAMP1 (NT) (A). After stripping, the membrane was reprobed with an antibody against the 39-kd subunit of the VATPase (B). As a positive control for the anti-NRAMP1 antibody, 5μg membrane proteins from U937 transfectants expressing the c-Myc–tagged NRAMP1 was included (lane 5). The positions and sizes (in kilodaltons) of the molecular weight markers are shown.$

Detection of NRAMP1 in human neutrophils granules by Western blotting.

An immunoblot of purified fractions (lane 1, α; lane 2, β1; lane 3, β2; and lane 4, γ; 25 μg of each fraction per lane) was probed with an antibody against NRAMP1 (NT) (A). After stripping, the membrane was reprobed with an antibody against the 39-kd subunit of the VATPase (B). As a positive control for the anti-NRAMP1 antibody, 5μg membrane proteins from U937 transfectants expressing the c-Myc–tagged NRAMP1 was included (lane 5). The positions and sizes (in kilodaltons) of the molecular weight markers are shown.

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