Fig. 1.
Fig. 1. Western blot of expressed wild-type and mutant FVIII and cleavage by thrombin. / The blot was probed using monoclonal antibody R8B12, which recognizes an epitope within the A2 domain. Lane designations are: 1 and 2, FVIIIWT and FVIIIaWT; 3 and 4, FVIIISer558Phe and FVIIIaSer558Phe; 5 and 6, FVIIIVal559Ala and FVIIIaVal559Ala; 7 and 8, FVIIIAsp560Ala and FVIIIaAsp560Ala; 9 and 10, FVIIIArg562Ala and FVIIIaArg562Ala; 11 and 12, FVIIIGln565Arg and FVIIIaGln565Arg. Lane m shows the positions for the protein markers (Mr is ×10−3).

Western blot of expressed wild-type and mutant FVIII and cleavage by thrombin.

The blot was probed using monoclonal antibody R8B12, which recognizes an epitope within the A2 domain. Lane designations are: 1 and 2, FVIIIWT and FVIIIaWT; 3 and 4, FVIIISer558Phe and FVIIIaSer558Phe; 5 and 6, FVIIIVal559Ala and FVIIIaVal559Ala; 7 and 8, FVIIIAsp560Ala and FVIIIaAsp560Ala; 9 and 10, FVIIIArg562Ala and FVIIIaArg562Ala; 11 and 12, FVIIIGln565Arg and FVIIIaGln565Arg. Lane m shows the positions for the protein markers (Mr is ×10−3).

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