Differentiation characteristics of M1, M1Neo, and M1NF-YA DN cell in mass culture.

Cells were collected 3 days following IL-6 stimulation (10 ng/mL), and morphologic differentiation was determined by counting at least 300 cells on May-Grünwald Giemsa–stained cytospin smears and scoring the proportion of immature blast cells, cells at the intermediate monocytic differentiation, and mature macrophages as detailed in “Materials and methods.” Percentage of cells expressing the macrophages specific cell surface marker F4/80 was determined by FACS analysis as detailed in “Materials and methods.” Values are averages of 3 independent experiments, and SDs are indicated. The SD of M1 blast cells is too small to be seen.