NF-YA DN reduces MyD gene induction by IL-6.

(A) NF-Y binding motifs in MyD gene promoter regions. Underlined bases indicate a match with the NF-Y consensus sequence. No match was found for MyD116 and H1₀. Position on the promoter is relative to the transcription start site, which has not been determined for MyD118. Pu, Purine residues. ¹Indicates the inverse sequence. (B) MyD expression in M1 control and M1NF-YA DN IL-6–treated cells. RNA was extracted from the cell lines, untreated or treated with IL-6 (100 ng/mL) for the indicated time points, and Northern blots were probed with the appropriate labeled cDNA fragments. Genes were categorized into MyD genes and housekeeping (HK) genes. Similar levels of MyD gene expression were observed for 2 additional M1NF-YA DN clones. As detailed in Figure 3D, the level of MyD gene expression in M1NF-YA DN and M1Neo cells was quantitated and expressed as a ratio. To assess if NF-YA DN suppresses MyD similarly to DNA binding, the suppression ratios of each were compared (MyD sup/NF-Y binding sup).