Analysis of multilineage colonies from flow-sorted CD34⁺CD19⁻ cells.

The upper left photos show a CFU-G together with a BFU-E (A) and a multilineage CFU-GEMM (B) in methylcellulose cultures initiated with purified CD34⁺CD19⁻ cells from patient nos. 589/97 (A) and 1523/00 (B). A Pappenheim-stained smear of the CFU-GEMM shows the presence of granulocytic (g), macrophage (m), and erythroid cells (C). Original magnification A-B, × 50; C, × 1000. A representative RT-PCR analysis of 18 colonies from patient no. 1523/00 and 8 colonies from patient no. 589/97 shows that CFU derived from sorted CD34⁺CD19⁻ cells lack expression of TEL/AML1 (D).