Dual FISH analysis of BM smears from MDS cases with both del(5q) and trisomy 8.

MGG-stained BM smears from 3 MDS cases known to have both del(5q) and trisomy 8 were investigated by dual FISH analysis for presence of the 2 abberations in the granulocytic (A) and erythroid (B) lineages (as described in Table 5). Data are presented as percentage of total cells within each lineage that had a normal chromosome 5 and 8 status or different combinations of 5q deletion and/or trisomy 8 (cutoff value at 2%-3% marked with horizontal lines). *See Table 5. Also shown is an example of MGG-stained erythroid cells (C) from patient no. 5 and the subsequent dual FISH analysis of the same cells (D). Note that all cells have both del(5q) and trisomy 8. Original magnification approximately × 1000.