Fig. 4.
Fig. 4. The diagnostic 235-bp insertion within the ashRab27a transcript is found in homozygous form in both C3H/HeSnJash/ash and a/a, ash/ash mutant mice. / RT-PCR amplification of kidney RNA produces a 710-bp product in normal C3H/HeSnJ mice and a 945-bp product in both ashen mutants. The 1 Kb+ size markers (GibcoBRL, Carlsbad, CA) and PCR products were detected by ethidium bromide staining.

The diagnostic 235-bp insertion within the ashRab27a transcript is found in homozygous form in both C3H/HeSnJash/ash and a/a, ash/ash mutant mice.

RT-PCR amplification of kidney RNA produces a 710-bp product in normal C3H/HeSnJ mice and a 945-bp product in both ashen mutants. The 1 Kb+ size markers (GibcoBRL, Carlsbad, CA) and PCR products were detected by ethidium bromide staining.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal