Vitamin C inhibits GM-CSF–dependent cell proliferation.

MO7e cells incubated for 30 minutes with or without 500 μM DHA were grown in a 96-well tissue culture plate in the presence or absence of 400 pM GM-CSF for 24 hours or 48 hours. Proliferation was measured by colorimetric assay. WST-1 was added and 3 hours later the absorbance was measured (450 nm-690 nm) by ELISA reader.