Inhibition of Ca²⁺ mobilization suppresses SCF-induced cell migration.

(A) BAF/WT cells were preincubated with the indicated concentrations of BAPTA-AM for 30 minutes. Then, cell migration was induced by 30 ng/mL SCF in the presence of BAPTA-AM in both chambers. Data are shown as percentages of cells in lower chamber to the whole applied cells. The results are shown as the means ± SD of triplicate cultures. (B) Inhibition of Ca²⁺ influx of BAF3/KIT cells with BAPTA-AM. The cells were preincubated with BAPTA-AM for 30 minutes at 37°C. The intracellular Ca²⁺ concentration was measured as described above. Similar results were obtained from 3 independent experiments.