hIAN1 mRNA expression pattern.

(A) Expression of hIAN1 mRNA in various human tissues. Clontech poly A⁺ Multiple Tissues Blot was hybridized with an hIAN1 probe. Each lane contains 2 μg poly A⁺ RNA. Lane 1, spleen; lane 2, thymus; lane 3, prostate; lane 4, testis; lane 5, ovary; lane 6, small intestine; lane 7, colon; lane 8, peripheral blood lymphocytes. The amounts of RNA loaded and transferred to the membrane were assessed by a GAPDH hybridization. (B) Expression of hIAN1 mRNA in mature hematopoietic cells. RT-PCR analysis of hIAN1 mRNA expression was performed on B lymphocytes (lane 1), T lymphocytes (lane 2), mature erythroid cells (lane 3), mature myeloid cells (lane 4), and mature megakaryocytic (lane 5) cells. Jurkat and Jurkat-ΔCOOH cells (lanes 6 and 7) were used as controls. The amount of cDNA used for PCR was normalized relative to the levels of the S14 gene product.