Fig. 1.
Fig. 1. hIAN1 mRNA expression in Jurkat and Jurkat-ΔCOOH. / (A) Growth analysis of Jurkat and Jurkat-ΔCOOH. The cells were cultured in RPMI 1640 containing 10% FCS and counted daily for 5 days. Viable cells represented trypan blue–negative cells that do not display any characteristic features of cells undergoing apoptosis. ● indicates Jurkat; ■, Jurkat-ΔCOOH. (B) Northern blot analysis of hIAN1 mRNA expression in Jurkat and Jurkat-ΔCOOH. The blot contained 5 μg total RNA isolated from Jurkat (odd lanes) and Jurkat-ΔCOOH (even lanes) at days 0 (lanes 1 and 2), 1 (lanes 3 and 4), and 3 (lanes 5 and 6) of culture and was hybridized with a radiolabeled 441-bpDpnII-DpnII fragment of the hIAN cDNA. The amount of RNA loaded in each lane was assessed by 18S and 28S staining.

hIAN1 mRNA expression in Jurkat and Jurkat-ΔCOOH.

(A) Growth analysis of Jurkat and Jurkat-ΔCOOH. The cells were cultured in RPMI 1640 containing 10% FCS and counted daily for 5 days. Viable cells represented trypan blue–negative cells that do not display any characteristic features of cells undergoing apoptosis. ● indicates Jurkat; ■, Jurkat-ΔCOOH. (B) Northern blot analysis of hIAN1 mRNA expression in Jurkat and Jurkat-ΔCOOH. The blot contained 5 μg total RNA isolated from Jurkat (odd lanes) and Jurkat-ΔCOOH (even lanes) at days 0 (lanes 1 and 2), 1 (lanes 3 and 4), and 3 (lanes 5 and 6) of culture and was hybridized with a radiolabeled 441-bpDpnII-DpnII fragment of the hIAN cDNA. The amount of RNA loaded in each lane was assessed by 18S and 28S staining.

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