Effect of CD40L expression on expression of costimulatory molecules and MHC class II molecules in the CD40⁺ plasmacytoma cell lines.

CD40L expression does not up-regulate expression of costimulatory molecules and MHC class II molecules in either of the CD40⁺plasmacytoma cell lines. MPC-11 (panels A-B) and S107 (panels C-D) cells were cocultured in vitro with either CL7.1/neo (panels A,C) or CL7.1/mCD40L (panels B,D) fibroblasts. Cells were analyzed after 24, 48, and 72 hours. The expression of CD80 and CD86 costimulatory molecules as well as of MHC class II molecules is unaffected by transgenic CD40L. Data are representative of 3 independent experiments on each line.