Increased erythroid cells and decreased myeloid cells in cultures of p210-transduced CB cells.

Representative FACS profiles of cells from 7-day-old serum-free cultures of MIG (left panel) and p210-transduced (right panel) (GFP⁺) CD34⁺ CB cells maintained in FL, SF, IL-3, IL-6, and G-CSF but no added epo illustrating the differences seen in granulopoietic (CD15) and erythroid (glycophorin A) marker expression. The reduced level of GFP expression seen here is due to the fact that the cells were cultured in the absence of serum as shown by the fact that subsequent addition of serum increased the average level of GFP fluorescence several-fold (data not shown).