Altered differentiation of cultured p210-transduced CB cells as shown by Wright staining.

Representative cytospins prepared from 7 day-old serum-free cultures of MIG (A) and p210-transduced (B) (GFP⁺) CD34⁺ CB cells maintained in FL, SF, IL-3, IL-6, and G-CSF but no added epo. These show the prevalence of recognizable granulocyte (metamyelocyte, band forms) and erythroid (basophilic erythroblasts) precursors, respectively, in the cultures initiated with these 2 types of cells (× 400 magnification). At later times, large numbers of polychromatophilic erythroblasts were also seen in the cultures of p210-transduced cells.