Expression of mPILSAP.

(A) MSS31 cells were preincubated for 24 hours in 0.1% FCS/αMEM and then stimulated with 100 ng/mL VEGF for the indicated period. (B) After preincubation, MSS31 cells were stimulated with the indicated concentrations of VEGF for 4 hours. Total RNA was obtained and Northern blot analysis performed. As a control, 28S RNA in the ethidium bromide–stained gel is also shown. (C) Total RNAs were obtained from Flk-1⁺/VE-cadherin⁻ and Flk-1⁺/VE-cadherin⁺ cells. RT-PCR was performed as described in “Materials and methods.” Mouse G3PDH was used as internal control. (D) After preincubation, MSS31 cells were stimulated with the indicated concentrations of VEGF for 8 hours. Western blot analysis was performed as described in “Materials and methods.”