Divergent effects of LPS on TLR4 mRNA and surface protein levels.

(A) Surface expression of TLR4 in human monocytes exposed to LPS (100 ng/mL) or medium alone (Ctr) for 24 hours. The insert shows TLR4 mRNA levels after 4 hours of treatment (see also references 20 and 24). (B) TLR4 surface expression in human monocytes exposed for 4 hours to LPS (100 ng/mL) or CHX (10 μg/mL), or to a combination of the 2. (C) TLR4 surface expression in human monocytes exposed for 24 hours to the combination of IFNγ (500 U/mL) and LPS (100 ng/mL). MCF is indicated for each treatment. Dotted line indicates irrelevant control mAb.