HTLV-I–positive cells produce VEGF and bFGF.

(A,B) Analysis of VEGF and bFGF mRNA isoforms. HTLV-I–transformed cells (HuT-102, MT-2, C8166, C91PL) and HTLV-I–negative cells (Molt-4, Jurkat, CEM) were analyzed for VEGF and bFGF mRNA expression by RT-PCR using 100 ng total RNA (A). bFGF mRNA expression was also assessed by RT-PCR using 500 ng total RNA (B). (C,D) Detection of cell-associated VEGF and bFGF proteins. Total cell extracts from HTLV-I–transformed cells, HTLV-I–negative cells, and endothelial cells (ECV-304) were analyzed by Western blot using anti-VEGF– (C) and anti-bFGF– (D) specific antibodies. Antiactin and anti-GAPDH antibodies were used for assessment of protein loading.