Fig. 5.
![Fig. 5. Human CD33 cytoplasmic tail is phosphorylated by purified human PKC in vitro. / Coomassie-stained SDS-PAGE (A) and autoradiograph of the same gel (B). The cytoplasmic tail of CD33 was expressed as a GST fusion protein inE coli, purified, and [32P]-orthophosphate PKC labeled under activating conditions (+ indicates Ca++ and lipids; −, no Hepes and EGTA) and nonactivating conditions (+, Hepes and EGTA; −, no Ca++ and lipids). PKC-mediated labeling of the GST-CD33 tail region is reduced under nonactivating conditions, proving phosphorylation by the purified human PKC. GST alone is not phosphorylated by PKC under these conditions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3188/6/h80922499005.jpeg?Expires=1724253762&Signature=nYI0nirGoj2mTXLXmb2LCk81RS80QBX-bmphrRICWo1QBfvaXGCz2Pgb3fz6yIDdyaKGbPaPjkSWuYHl29hiTfYDSuZOiDpIzeAQ2Z1ybLwmbEkNVBGExhMSTUrufP4MIDIlOmZr6QtJB-tnWHhQxKbZVWJOYD1wklaOhPljLUBrsUKnxQHTA2CQEyz7q2cmAyiZhpWFqJ~~CZN-zh5AZi7BVL6ijpgfAd4lpsLQcMOSr-pzeGtOxk8b5x7~-dBTKaY1Fsp6tSSu3mVnHtDBN2KVD4rvDp2kGgYW-q~IyOCAW0s8hO9DJKT2-ofI-tcSceAZF3MYQ4-uxP5CTZb7FQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Human CD33 cytoplasmic tail is phosphorylated by purified human PKC in vitro.
Coomassie-stained SDS-PAGE (A) and autoradiograph of the same gel (B). The cytoplasmic tail of CD33 was expressed as a GST fusion protein inE coli, purified, and [32P]-orthophosphate PKC labeled under activating conditions (+ indicates Ca++ and lipids; −, no Hepes and EGTA) and nonactivating conditions (+, Hepes and EGTA; −, no Ca++ and lipids). PKC-mediated labeling of the GST-CD33 tail region is reduced under nonactivating conditions, proving phosphorylation by the purified human PKC. GST alone is not phosphorylated by PKC under these conditions.
