Fig. 2.
![Fig. 2. CD33 phosphoamino acid analysis in U937 or HL60 cells detects only phosphoserine. / [32P]-orthophosphate-labeled CD33 from PMA-stimulated U937 (A) or HL-60 cells (B) was isolated by My9 adsorption and SDS-PAGE as described in “Materials and methods.” Following hydrolysis in 6 N HCl, the resulting phosphoamino acids were mixed with phosphoserine (pS), phosphothreonine (pT), and phosphotyrosine (pY) standards and analyzed by 2-dimensional high-voltage thin-layer cellulose chromatography. Samples were applied at (+). The radioactivity above the origin represents partially digested phosphopeptides, whereas that in the upper left corner represents free phosphate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3188/6/h80922499002.jpeg?Expires=1724253494&Signature=McV6A2VgBJQfXr~pUXG-1gqm62MCLoTsqMzzjObrt2YTzQV-bslw2OU52iOC~eaWIwOdz8zgqnQPRYBGaTw05WdvGHckNcGNUbjW4TjR6wkZPJmcq16IRHsOBG4Xa8xVl2jYEO3YYPO4R2ixctmsUJVrm3RxlwzFAQAiLd3EXyP3yho7yMZgtefdGcTiz~bRornc4Da4XjgXkrSu0tqq6puPNkCq6B6hSsv5XulEQnlDytvDdMup7KWXj3CL0vwSHJLjVexlmukF39eVG1CroyX7GAZqJwjaYTnuGHM5xSIXruVBmXm1JOFrvpqK7bZp2-gPbZn2VfmMl~sm4Kvb1g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD33 phosphoamino acid analysis in U937 or HL60 cells detects only phosphoserine.
[32P]-orthophosphate-labeled CD33 from PMA-stimulated U937 (A) or HL-60 cells (B) was isolated by My9 adsorption and SDS-PAGE as described in “Materials and methods.” Following hydrolysis in 6 N HCl, the resulting phosphoamino acids were mixed with phosphoserine (pS), phosphothreonine (pT), and phosphotyrosine (pY) standards and analyzed by 2-dimensional high-voltage thin-layer cellulose chromatography. Samples were applied at (+). The radioactivity above the origin represents partially digested phosphopeptides, whereas that in the upper left corner represents free phosphate.
