Fig. 1.
![Fig. 1. CD33 phosphorylation in myeloid cell lines is induced by phorbol esters. / U937 cells or HL-60 cells were labeled with [32P]-orthophosphate for 1 hour and then treated for 12 minutes with either PMA (18 nM), DMSO (1.5%), or control (0.001% DMSO) for 12 minutes. CD33 immunoprecipitates were isolated using My9, blotted onto nitrocellulose, and detected by fluorography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3188/6/h80922499001.jpeg?Expires=1724253459&Signature=RXfg10NJWUEAyz6oIA4U8viEBFNQNRP8tKncqjQn~lpcGOzj-El5~zH0aYA4ae4jsmdWc-ad2-6Zu0nP3sRw5r-qdGi2EVWyrOYvqDKruwaij~Fp9oWXcHJksf1Pt2Dj1YqIs2zzh9n8I1gwVxAB6ibL7Kpho-LEqQbJKY6zNs3gldOMF4mY0qxzn1u8OfNMHySs7njHE63vRC5wr~pdizvoSDBDCnWrHTLpfE6~ML8PVlPQ7ndmtA75v4n-K9~4xMLxCfccxbS3WzZS8ZZHyNYiEBKt26RpXc4-NyvBUPLCODjrpiKOTaaTkPpEZGwGQHiwFBvlCyAjza-TRUdU3g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD33 phosphorylation in myeloid cell lines is induced by phorbol esters.
U937 cells or HL-60 cells were labeled with [32P]-orthophosphate for 1 hour and then treated for 12 minutes with either PMA (18 nM), DMSO (1.5%), or control (0.001% DMSO) for 12 minutes. CD33 immunoprecipitates were isolated using My9, blotted onto nitrocellulose, and detected by fluorography.
