Depletion experiments.

PBMCs were sort-depleted of CD14, CD16, or CD19 cells and cultured in parallel with whole PBMC samples. The percentage of DCs within the whole PBMC culture (▴, continuous line), CD14-depleted (▾), CD16-depleted (□), and CD19-depleted (⋄) was evaluated over a 24-hour period (3 experiments shown). Analyses at each time point were performed in triplicates. Error bars show SEM (A). Dot plots demonstrating the Lin⁻ HLA-DR⁺ DC profiles within the different depleted cultures at the 4-hour incubation time point from one experiment are shown (B), and they are representative of the 3 separate experiments performed.