CD14⁺ myelomonocytes derived from intrathymic CD34^hiCD33^lo pro–T cells are DC1 precursors.

(A) Representative flow cytometry profile (shaded monoparametric histograms) of electronically gated CD13⁺CD14⁺and CD13⁺CD14^−/lo cell subsets (biparametric plot) derived by day 6 from CD34^hiCD33^loprecursors set up in multicytokine-supported cultures. Background fluorescence determined with isotype-matched irrelevant MoAbs is shown (unshaded histograms). (B) Bright-field images of CD14⁺(upper panel) and CD14^−/lo (lower panel) subsets sorted as shown in (A) after overnight culture (original magnification × 40), and flow cytometry analysis for the correlated expression of CD14 and CD13 (biparametric plots), and CD1a (shaded histograms) on their cellular progenies derived upon reculture for 3 additional days.