Catalytic activity of ZAP-70 is required for CXCL12-induced T-cell migration.

Jurkat KD12, FF18-22, and Y41-40 cell lines were added to the upper well of a Transwell chamber. Medium alone or medium plus CXCL12 (100 ng/mL) was added to the lower chamber. Transendothelial migration of the cells was measured as above. Expression of VSV-tagged ZAP-70 mutants was visualized by anti-VSV immunoblot analysis of cell lysates.