Transfection of a dominant-negative form of Vav1 decreases T-cell migration in the presence of CXCL12.

Jurkat-TAg cells were transfected with 30 μg of an empty vector pEFneo (used as a control) or plasmids encoding 3BP2-myc, Vav1-myc L213A, wild-type Vav1-myc, and Rac1 N17, together with 5μg pEGFP-N1 vector. Cells were cultured for 48 hours and were used in cell migration assays, as described in Figure 1A. Transfected GFP⁺ cells were sorted by FACS analysis.