CXCL12-induced T-cell transendothelial migration is blocked by the ZAP-70 inhibitor piceatannol.

(A) Jurkat cells were preincubated with the indicated concentrations of piceatannol for 3 hours at 37°C and then were added to the upper well of a Transwell chamber. Medium alone or medium plus CXCL12 (100 ng/mL) was added to the lower chamber. Transendothelial migration and quantification of cell migration were performed as above. (B) Jurkat cells were left untreated (control) or were preincubated with the PTK inhibitor herbimycin A (10 μM), the PI3 kinase inhibitor Ly294002 (20 μM), the G-protein inhibitor pertussis toxin (10 ng/mL), or the calmodulin inhibitor KN62 (40 μM) for 3 hours at 37°C. Transendothelial migration and quantification of cell migration was performed as in panel A.