Western blot analysis of HGF released after PMN degranulation: effect of DFP.

PMNs were incubated with 2 mM DFP (DFP⁺) or without DFP (DFP⁻) for 15 minutes at 4°C then stimulated with cytochalasin B and fMLP at 37°C; the supernatant was then subjected to Western blot analysis in reducing conditions and revealed with antihuman HGF monoclonal antibodies. These data are representative of one experiment of 3.