Fig. 2.
![Fig. 2. Messenger RNase protection analysis and ligand binding studies on transfected 32D/G-CSF-R cells. / (A) RNase protection analysis using probe P on 10 μg total mRNA from 4 distinct transfected 32D/G-CSF-R clones: 2 32D/G-CSF-R clones transfected with Cb2 exon-1B/exon-2 cDNA and 2 clones with control vector pLNCX. (B) Cb2 receptor density and receptor affinity for CP55940 on 2 Cb2 exon-1B/exon-2 clones and 2 empty vector control clones. Density and affinity were assessed by saturation radioligand receptor binding experiments using [3H]CP55940. Kd is added to the columns.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/8/10.1182_blood.v99.8.2786/5/h80822431002.jpeg?Expires=1723738979&Signature=iz9-277b5c7YT4m0FFlrYEXTiT5TpNIafsSI3KjIshM1Kad0FOw2bxRGHabvl05V9Y72xFA6U00J85LUQxd5uEpilUIM17JGGBb-C4quppPV0pa5HDArv0Rwh7IPn0LOX5LlInLbaJCm~C9xFYwdpFbajU0aywAwpulmJkeA-AGYETVmqegM0HytemORYGemHfJdR6X1vngo1z1LQmzGUPzTxmrNdSrTOKzCjiAe7UCcWq1ghz8XkThzjfV56gFugp5iRKxBqwad3Lqyz2hWVxacI6nU0lJkMLSsVp8joQhO31V8fBDpYvO~Xvm6S6xjt7PFmjK3dWD~MislCDGqdA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Messenger RNase protection analysis and ligand binding studies on transfected 32D/G-CSF-R cells.
(A) RNase protection analysis using probe P on 10 μg total mRNA from 4 distinct transfected 32D/G-CSF-R clones: 2 32D/G-CSF-R clones transfected with Cb2 exon-1B/exon-2 cDNA and 2 clones with control vector pLNCX. (B) Cb2 receptor density and receptor affinity for CP55940 on 2 Cb2 exon-1B/exon-2 clones and 2 empty vector control clones. Density and affinity were assessed by saturation radioligand receptor binding experiments using [3H]CP55940. Kd is added to the columns.
