Fig. 1.
![Fig. 1. Expression of distinct. / Cb2 mRNA splice variants and determination of receptor binding sites in mouse myeloid leukemia cell lines. (A) Genomic organization of mouse Cb2. The gray box represents the Cb2 protein-coding region. Two distinct splice variants have been identified: exon-1A/exon-2 and exon-1B/exon-2. The 249 nucleotide RNA probe (probe P) representing exon-1B/exon-2 that was used for RNase protection analysis is indicated. (B) RNase protection analysis on distinct myeloid cell lines using probe P (see panel A). (C) Cb2 receptor density and receptor affinity for CP55940 on the NFS cell lines was assessed by saturation radioligand receptor binding experiments using [3H]CP55940. Kdis added to the columns.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/8/10.1182_blood.v99.8.2786/5/h80822431001.jpeg?Expires=1723738805&Signature=oLGuOLzhhoYHHT0xVkfdWC1mcxudDlcRdwUyPJybHZVCtXZNrp2T0AeSDs8efntfHd7nGQ7q7NCTXCgF8rM7FjvMtVsgDypmsp3AOSlNTbWdjzpuNiE5m-BcEd9f5C9Rzjkn9rM3yHKXzUXs6I7Dw6-n4tHm0vO15W5Tb5Fla2sWCbJ-ubszGNjh8~TngqFe1FjkfpPDYx7J2lWhvG9610Obszwop6H68-fxQ6kGANpYPB3VRvXgBilDVa65ysRTln~tYgoXR7tfc0YNyQU9A-7MBLiI1inJrX2AoqzGbUyYNcx1KD09hI3d-hpi538Ux2njMyyZhna8otWNq5KjxA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of distinct
Cb2 mRNA splice variants and determination of receptor binding sites in mouse myeloid leukemia cell lines. (A) Genomic organization of mouse Cb2. The gray box represents the Cb2 protein-coding region. Two distinct splice variants have been identified: exon-1A/exon-2 and exon-1B/exon-2. The 249 nucleotide RNA probe (probe P) representing exon-1B/exon-2 that was used for RNase protection analysis is indicated. (B) RNase protection analysis on distinct myeloid cell lines using probe P (see panel A). (C) Cb2 receptor density and receptor affinity for CP55940 on the NFS cell lines was assessed by saturation radioligand receptor binding experiments using [3H]CP55940. Kdis added to the columns.
