Reduced in vivo expression of donor T cells depleted from host reactivity by AICD.

Shown by flow cytometry analysis is a very low expansion of allogeneic CD4⁺ or CD8⁺ T cells (H-2^k) depleted of alloreactive responders (B) in contrast to significantly increased populations of untreated allogeneic donor CD4⁺and CD8⁺ T cells (A) in F₁ hosts 10 days after transfer. Monitoring the expansion of donor-derived T cells was performed on CD4⁺ and CD8⁺ T cells isolated from spleen and lymph node suspension by immunomagnetic separation and stained for H-2k^k and H-2D^d to discriminate single-positive donor-derived (H-2^k) T cells from F₁-specific (H-2^kxd) Tlymphocytes.