Reduced proliferative responses of alloreactive T cells upon induction of AICD by agonistic anti-CD95 mAb.

Strong reduction of alloreactivity by CD4⁺ and CD8⁺ responders upon induction of CD95-mediated AICD by agonistic mAb to CD95 in a dose-dependent fashion. Naive T cells from C3H/He (H-2^k) mice were purified into CD4⁺ and CD8⁺ subpopulations by immunomagnetic separation using MACS. Following stimulation with irradiated allogeneic splenic stimulators from BALB/c (H-2^d) in an MLC over 6 days in the absence (▪) or presence of 1 μg/mL (●) or 5 ng/mL (♦) of agonistic anti-CD95 mAb (Jo2), proliferation of T cells was determined by ³H-thymidine uptake for the last 12 hours. Addition of a 1 μg/mL isotype-matched hamster-Ig control antibody (○) during culture was performed as specificity control. Syngeneic controls (■) are shown without induction of AICD. Results are expressed as mean ± SD of triplicate wells and depict 1 of at least 5 representative experiments. (A) Proliferative response by allogeneic CD4⁺responders. (B) Proliferative response by allogeneic CD8⁺T cells.